Reserch Paper no.32

Heat-inactivated Weissella cibaria CMU downregulates the mRNA expression of proinflammatory cytokines and matrix metalloproteinases in Porphyromonas gingivalis LPS-stimulated human gingival fibroblasts

Source : Korean J Oral Anatomy. 2022; 43(1):75-85. / KCI / In vitro
Authors : Min-Jeong Kim, Yong-Ouk You, Hyun-Jin Kim


Weissella cibaria CMU (oraCMU) is reported as one of the probiotics that promote oral health. Various periodontal pathogens, including Porphyromonas gingivalis (P. gingivalis), can cause periodontal tissue de- struction and periodontal disease. P. gingivalis infection in human gingival fibroblasts (HGFs) induces the ex- pression of proinflammatory cytokines and enhances the production of matrix metalloproteinases (MMPs). The present study investigated the inhibitory effects of heat-inactivated oraCMU on the mRNA expression of proin- flammatory cytokines and MMPs in P. gingivalis LPS-stimulated HGFs. Cell viability was performed using the MTS assay. The mRNA expression of proinflammatory cytokines, such as interleukin-1β (IL-1β) and, IL-6, tumor necrosis factor-α (TNF-α), and MMPs, such as MMP-1 and MMP-3, was examined by reverse transcrip- tion-quantitative PCR (RT-qPCR). Heat-inactivated oraCMU dose-dependently inhibited the mRNA expression of IL-1β, IL-6, and TNF-α in P. gingivalis LPS-stimulated HGFs. Also, the mRNA expression of MMP-1 and MMP-3 dose-dependently decreased. These results suggest that heat-inactivated oraCMU could be an effective probiotic for preventing periodontitis.

Keywords: Weissella cibaria CMU, Matrix metalloproteinase, proinflammatory cytokines, Porphyromonas gingivalis, human gingival fibroblasts  


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