Research Paper no.35

In Vitro Effects of Weissella cibaria CMU and CMS1 on Receptor Activator of NF-κB Ligand (RANKL)-Induced Osteoclast Differentiation

Source:  J. Funct. Biomater. 2024,15,65 
Authors: Keun-Yeong Park, Jeong-Ae Park, and Mi-Sun Kang
Published: March 8, 2024

Keywords: Weissella cibaria; RAW 264.7 cells; osteoclast; bone resorption; periodontitis 

High activity of bone-resorbing cells, called osteoclasts, can lead to bone loss in gum disease. The effects of Weissella cibaria bacteria strains CMU and CMS1 on this type of bone loss are not fully understood. In our study, we explored whether dead (heat-killed) W. cibaria strains CMU and CMS1, or substances from these bacteria (cell-free supernatants), can prevent bone-resorbing cells from forming and breaking down bone. We treated bone-resorbing cells with a substance that usually increases their activity (RANKL) and tested the effects of the dead bacteria and their substances.

Our findings show that both the dead bacteria and their substances significantly reduced the formation of bone-resorbing cells and their activity, as well as actual bone damage, compared to the control group treated only with RANKL. The dead bacteria also affected the activity of specific genes related to bone resorption. Interestingly, when we prevented the dead bacteria from directly interacting with the bone-resorbing cells, the bacteria's ability to reduce bone resorption protein levels was lost. This suggests that the direct contact between the dead bacteria and the cells is crucial for their protective effect against bone loss.

Figure 1. 

Effects of heat-killed W. cibaria CMU (HK-oraCMU), CMS1 (HK-oraCMS1), or the cell-free FiHguKr-eo1r.aECffMecSt1s otfrehaetamt-keinlltedgrWou. cpibsadrioa sCeM-dUep(HenKd-oernatClyM(Up )<, C0M.05S)1 (FHiKgu-orreaC2JM).SH1)i,gohr tchoencceelln-ftreae- ssupeerrnattanttss(CFSs)ofW.cibariaCMU((CFFSS--oorraaCMU))aannddCCMMSS11(C(CFSF-So-roarCaCMMS1S)1o)nonthtehveivabiaibliitlyitoyf tions of HK-oraCMU and HK-oraCMS1 (MOI = 1000) reduced TRAP activity by 46.0% oRfARWAW2642.674c.7elclse.llAs.llAglrlogurposusphsoswheodwneod sntoatissttaitciasltliycaslilgynsifiigcnainfitcdainfftedreiffnceeresnbceetswbeetnwgereonupgsro(pup>s0(.p05>). 

This study explored the effects of two specific strains of heat-killed (HK) Weissella cibaria bacteria, named CMU and CMS1, and their cell-free byproducts (CFSs) on bone loss associated with periodontitis.

"These findings suggest that dead Weissella cibaria bacteria might be useful in preventing bone loss in periodontitis more effectively than their metabolic byproducts, by stopping the formation of bone-destructive cells through direct cell interaction."

Overall, our results indicate that dead W. cibaria bacteria are more effective than their released substances in preventing bone loss associated with gum disease, highlighting their potential as therapeutic agents in the fight against this condition.

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