Abstract 

This study aimed to investigate the anti-inflammatory effects of Weissella cibaria CMU (oraCMU), a probiotic strain isolated from the saliva of healthy children, on RAW 264.7 cells stimulated by Porphyromonas gingivalis (P. gingivalis). RAW 264.7 cells were treated with oraCMU at multiplicities of infection (MOI) of 0.1, 1, 10, and 100, and cell viability was assessed using an MTS assay. Nitric oxide (NO) production was measured using NO assay after treating RAW 264.7 cells with oraCMU at MOIs of 0.1, 1, and 10, followed by stimulation with P. gingivalis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure mRNA levels of the pro-inflammatory cytokines interleukin (IL)-1β and IL-6. To investigate the signaling pathway of inflammatory transcription factor expression, Western blotting was performed to analyze the nuclear translocation of NF-κB and clarify the anti-inflammatory mechanism of oraCMU. The MTS assay showed no significant changes in cell viability at MOIs of 0.1, 1, 10, and 100. NO assay demonstrated that oraCMU treatment inhibited NO production in P. gingivalis-stimulated RAW 264.7 cells. Additionally, qRT-PCR analysis revealed reduced mRNA expression of IL-1β and IL-6 in P. gingivalis-stimulated RAW 264.7 cells treated with oraCMU. Western blot analysis further confirmed that oraCMU treatment inhibited NF-κB nuclear translocation. These findings suggest that oraCMU exerts anti-inflammatory effects on P. gingivalis-stimulated RAW 264.7 cells and may be a potential candidate for the development of food and pharmaceutical products for periodontitis treatment. 

Keywords: Porphyromonas gingivalis, periodontitis, anti-inflammatory, oraCMU, RAW 264.7 cellsn printer took a galley of type and scrambled it to make a type specimen book. 

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